Rabbit Polyclonal Antibody to ARPC1A
货号:
P33855
别名:
Actin-related protein 2/3 complex subunit 1A, SOP2-like protein, ARPC1A, SOP2L
应用:
WB,IHC,IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
Q92747
规格:
目录价
在线咨询
Description |
---|
ARPC1A is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. |
Specification |
|
---|---|
Aliases | Actin-related protein 2/3 complex subunit 1A, SOP2-like protein, ARPC1A, SOP2L |
Entrez GeneID | 10552 |
Swissprot | Q92747 |
WB Predicted band size | 41.6kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This ARPC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human ARPC1A. |
Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
---|---|
WB | 1/1000 |
IHC | 1/100-1/500 |
IF | 1/10-1/50 |
FCM | 1/10-1/50 |
Product Image
-
Anti-ARPC1A Antibody (C-term) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 42 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
-
Immunofluorescence analysis of ARPC1A Antibody (C-term) with hela cells. 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
-
Flow cytometric analysis of WiDr cells using ARPC1A Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
-
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.