Rabbit Polyclonal Antibody to IARS2
货号：
P33871
别名：
Isoleucine--tRNA ligase, mitochondrial, Isoleucyl-tRNA synthetase, IleRS, IARS2
应用：
WB,IHC
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q9NSE4
规格：
目录价
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Description |
|---|
IARS2 belongs to the class-I aminoacyl-tRNA synthetase family. Members of class I have two highly conserved sequence motifs. They aminoacylate at the 2'-OH of an adenosine nucleotide, and are usually monomeric or dimeric (one or two subunits, respectively). Both classes of aminoacyl-tRNA synthetases are multidomain proteins. The catalytic domains of all the aaRSs of a given class are found to be homologous to one another, while class I and class II aaRSs are unrelated to one another. The class I aaRSs have the ubiquitous Rossmann fold and have the antiparallel beta-strands architecture while the class II aaRSs have a unique fold made up of antiparallel beta-strands. |
Specification |
|
|---|---|
| Aliases | Isoleucine--tRNA ligase, mitochondrial, Isoleucyl-tRNA synthetase, IleRS, IARS2 |
| Entrez GeneID | 55699 |
| Swissprot | Q9NSE4 |
| WB Predicted band size | 113.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This IARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 600-628 amino acids from the Central region of human IARS2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
Product Image
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All lanes : Anti-IARS2 Antibody (Center) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 114 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IARS2 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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