Mouse Monoclonal Antibody to RYK
货号：
P33893
别名：
Tyrosine-protein kinase RYK, 2.7.10.1, RYK, JTK5A
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
P34925
规格：
目录价
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Description |
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May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development. |
Specification |
|
|---|---|
| Aliases | Tyrosine-protein kinase RYK, 2.7.10.1, RYK, JTK5A |
| Entrez GeneID | 6259 |
| Swissprot | P34925 |
| WB Predicted band size | 67.8kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RYK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 260-565 amino acids from human RYK. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
Product Image
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Anti-RYK Antibody at 1:1000 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing A431 cells stained with P33893(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33893, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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