Mouse Monoclonal Antibody to RAB1B
货号：
P33891
别名：
Ras-related protein Rab-1B, RAB1B
应用：
WB,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q9H0U4
规格：
目录价
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Description |
|---|
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1B regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum. |
Specification |
|
|---|---|
| Aliases | Ras-related protein Rab-1B, RAB1B |
| Entrez GeneID | 81876 |
| Swissprot | Q9H0U4 |
| WB Predicted band size | 22.2kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RAB1B antibody is generated from a mouse immunized with a recombinant protein between 1-201 amino acids from human RAB1B. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-RAB1B Antibody at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: U-20S whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing A431 cells stained with P33891(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33891, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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