Rabbit Polyclonal Antibody to SMURF2
货号：
P33924
别名：
E3 ubiquitin-protein ligase SMURF2, hSMURF2, 632-, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2
应用：
WB,IHC,IF
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q9HAU4
规格：
目录价
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Description |
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SMURF2 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with SMAD1, SMAD2 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. It enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level. |
Specification |
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| Aliases | E3 ubiquitin-protein ligase SMURF2, hSMURF2, 632-, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2 |
| Entrez GeneID | 64750 |
| Swissprot | Q9HAU4 |
| WB Predicted band size | 86.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SMURF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 702-731 amino acids from the C-terminal region of human SMURF2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
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|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
| IF | 1/10-1/50 |
Product Image
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Anti-SMURF2 Antibody (C-term) at 1:2000 dilution + C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-SMURF2 Pab (Cat. #P33924) in 293 cell line lysate (35ug/lane). SMURF2(arrow) was detected using the purified Pab
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Hippocampal neurons were fixed at stage 3, stained with anti-Smurf2 (red) and anti-Kinesin-2 (green) antibodies, and analyzed by confocal microscopy. The panels show single confocal planes. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268)
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Hippocampal neurons were transfected 2 h after plating with expression vectors for EGFP, EGFP-tagged Par3-4N/2, Par3-PDZ2, Par3-PDZ3, Smurf2-HECT (HECT), Smurf2-HECT-C716A (HECT CA), and shRNA directed against mPar3 (Par3 RNAi), or vectors for the anti-Par3 shRNA and human Myc-Par3 (RNAi + h Par3) (green). Transfected cells were analyzed at 3 d.i.v. by staining with an anti-Smurf2 antibody (red). Axons are marked by arrowheads. The marked growth cones are shown at a higher magnification. Scale bars, 40 and 10 ?. (J. Biol. Chem. 2007 Nov 30;282(48):35259-35268)
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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