Rabbit Polyclonal Antibody to PIGC
货号：
P33958
别名：
Phosphatidylinositol N-acetylglucosaminyltransferase subunit C, 2.4.1.198, Phosphatidylinositol-glycan biosynthesis class C protein, PIG-C, PIGC, GPI2
应用：
WB,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q92535
规格：
目录价
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Description |
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Part of the complex catalyzing the transfer of N- acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol, the first step of GPI biosynthesis. |
Specification |
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| Aliases | Phosphatidylinositol N-acetylglucosaminyltransferase subunit C, 2.4.1.198, Phosphatidylinositol-glycan biosynthesis class C protein, PIG-C, PIGC, GPI2 |
| Entrez GeneID | 5279 |
| Swissprot | Q92535 |
| WB Predicted band size | 33.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PIGC antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 264-294 amino acids from human PIGC. |
Application |
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| WB | 1/1000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-PIGC Antibody (C-Term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing K562 cells stained with P33958 (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33958, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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