Rabbit Polyclonal Antibody to PTTG2
货号：
P33994
别名：
Securin-2, Pituitary tumor-transforming gene 2 protein, PTTG2
应用：
WB,IF
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q9NZH5
规格：
目录价
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Specification |
|
|---|---|
| Aliases | Securin-2, Pituitary tumor-transforming gene 2 protein, PTTG2 |
| Entrez GeneID | 10744 |
| Swissprot | Q9NZH5 |
| WB Predicted band size | 22.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PTTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the Central region of human PTTG2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IF | 1/10-1/50 |
Product Image
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Anti-PTTG2 Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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PTTG2 Antibody (Center) (Cat. #P33994) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PTTG2 antibody detected the PTTG2 protein (arrow).
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Fluorescent confocal image of MCF-7 cell stained with PTTG2 Antibody (Center)(Cat#P33994).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTTG2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). PTTG2 immunoreactivity is localized to Cytoplasm significantly.
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