Rabbit Polyclonal Antibody to LEO1 (N-term)
货号：
P34019
别名：
RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, LEO1, RDL
应用：
WB
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q8WVC0
规格：
目录价
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Description |
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The PAF1 complex is a multifunctional complex. The PAF1 complex interacts with POLR2A. It may be involved in both initiation and elongation, histone methylation and RNA processing. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. |
Specification |
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|---|---|
| Aliases | RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, LEO1, RDL |
| Entrez GeneID | 123169 |
| Swissprot | Q8WVC0 |
| WB Predicted band size | 75.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This LEO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-159 amino acids from the N-terminal region of human LEO1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000 |
Product Image
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All lanes : Anti-Leo1 Antibody (N-term) at 1:1000-1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-Leo1 Antibody (N-term) (Cat. #P34019) in HL60 cell line lysates (35ug/lane). Leo1(arrow) was detected using the purified Pab.
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Western blot analysis of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term) (Cat.#P34019). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2) (Origene Technologies).
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