Rabbit Polyclonal Antibody to beta Tubulin
货号：
P34036
别名：
Tubulin beta-5 chain, Tubb5
应用：
WB,IHC,FCM
反应种属：
Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P99024
规格：
目录价
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Description |
|---|
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
Specification |
|
|---|---|
| Aliases | Tubulin beta-5 chain, Tubb5 |
| Entrez GeneID | 22154 |
| Swissprot | P99024 |
| WB Predicted band size | 49.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse, Rat |
| Immunogen | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 46-78 amino acids from human. |
Application |
|
|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-beta Tubulin at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: L6 whole cell lysate Lane 3: mouse brain lysate Lane 4: mouse spleen whole cell lysate Lane 5: mouse thymus lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing C2C12 cells stained with P34036(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34036, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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P34036 staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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