Rabbit Polyclonal Antibody to TPH1 (N-term)
货号:
P34046
别名:
Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH1, TPH, TPRH, TRPH
应用:
WB,IHC,IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P17752
规格:
目录价
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Description |
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TPH1 is a member of the aromatic amino acid hydroxylase family. This protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. |
Specification |
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|---|---|
| Aliases | Tryptophan 5-hydroxylase 1, Tryptophan 5-monooxygenase 1, TPH1, TPH, TPRH, TRPH |
| Entrez GeneID | 7166 |
| Swissprot | P17752 |
| WB Predicted band size | 51.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TPH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human TPH1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 |
| IHC | 1/100-1/500 |
| IF | 1/10-1/50 |
| FCM | 1/10-1/50 |
Product Image
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Anti-TPH1 Antibody (N-term) at 1:500 dilution + 293 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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Confocal immunofluorescent analysis of TPH1 Antibody (N-term)(Cat#P34046) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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Flow cytometric analysis of widr cells using TPH1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Formalin-fixed and paraffin-embedded human brain tissue with TPH1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.





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