Rabbit Polyclonal Antibody to RFC2 (N-term)
货号：
P34042
别名：
Replication factor C subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40, RFC2
应用：
WB,IHC,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P35250
规格：
目录价
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Description |
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The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. RFC2 is the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of RFC2 gene has been associated with Williams syndrome. |
Specification |
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|---|---|
| Aliases | Replication factor C subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40, RFC2 |
| Entrez GeneID | 5982 |
| Swissprot | P35250 |
| WB Predicted band size | 39.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RFC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human RFC2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
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All lanes : Anti-RFC2 Antibody (N-term) at 1:1000 dilution Lane 1: K562 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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RFC2 Antibody (N-term) (Cat. #P34042) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Rat lung, taken with 40x objective. Immunohistochemistry with RFC2 Antibody (N-term)(Cat.#P34042), 1:200 dilution,counter stained with Hematoxylin. Positive cells identified with arrows. Av-Alveoli, Br-Bronchus, Pa-Pulmonary Artery. (Provided by Hirotaka Ata,University of South Alabama, Dept of Biochem and Mol Biol)
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