Rabbit Polyclonal Antibody to PIAS3 (N-term)
货号：
P34096
别名：
E3 SUMO-protein ligase PIAS3, 632-, Protein inhibitor of activated STAT protein 3, PIAS3
应用：
WB,IHC
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q9Y6X2
规格：
目录价
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Description |
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PIAS3 is a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor, catalyzing the covalent attachment of a SUMO protein to specific target substrates. PIAS3 plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. |
Specification |
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| Aliases | E3 SUMO-protein ligase PIAS3, 632-, Protein inhibitor of activated STAT protein 3, PIAS3 |
| Entrez GeneID | 10401 |
| Swissprot | Q9Y6X2 |
| WB Predicted band size | 68.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PIAS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-126 amino acids from the N-terminal region of human PIAS3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
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|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
Product Image
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Anti-PIAS3 Antibody (N-term) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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