Rabbit Polyclonal Antibody to ASCL1 (Achaete-scute homolog 1) (C-term D220)
货号：
P34152
别名：
Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46, ASCL1, ASH1, BHLHA46, HASH1
应用：
WB
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P50553
规格：
目录价
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Description |
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ACSL1, alternatively titled Hash1 or Mash1, is a member of the basic helix-loop-helix (BHLH) family of transcription factors. It activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. ACSL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. The protein is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers. The presence of a CAG repeat in the gene suggests it may also play a role in tumor formation. |
Specification |
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| Aliases | Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46, ASCL1, ASH1, BHLHA46, HASH1 |
| Entrez GeneID | 429 |
| Swissprot | P50553 |
| WB Predicted band size | 25.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ASCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ASCL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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| WB | 1/1000 |
Product Image
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All lanes : Anti-Ascl1 (Human C-term) at 1:1000 dilution Lane 1: human brain lysate Lane 2: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-Ascl1 Antibody (C-term D220) (Cat. #P34152) in Hela cell line lysates (35ug/lane). Ascl1(arrow) was detected using the purified Pab (1:160 dilution).
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Western blot analysis of Ascl1 (arrow) using rabbit polyclonal Ascl1 Antibody (Human C-term) (Cat.#P34152). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Ascl1 gene (Lane 2) (Origene Technologies).
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