Rabbit Polyclonal Antibody to PCSK1
货号：
P34185
别名：
Neuroendocrine convertase 1, NEC 1, 3.4.21.93, Prohormone convertase 1, Proprotein convertase 1, PC1, PCSK1, NEC1
应用：
WB,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P29120
规格：
目录价
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Description |
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Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin. |
Specification |
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|---|---|
| Aliases | Neuroendocrine convertase 1, NEC 1, 3.4.21.93, Prohormone convertase 1, Proprotein convertase 1, PC1, PCSK1, NEC1 |
| Entrez GeneID | 5122 |
| Swissprot | P29120 |
| WB Predicted band size | 84.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PCSK1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 682-715 amino acids from human PCSK1. |
Application |
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|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-PCSK1 Antibody (C-Term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A549 whole cell lysate Lane 3: PC-12 whole cell lysate Lane 4: mouse brain lysate Lane 5: NCI-H460 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing A549 cells stained with P34185(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34185, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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