Rabbit Polyclonal Antibody to FTH1
货号：
P34205
别名：
Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein, Ferritin heavy chain, N-terminally processed, FTH1, FTH, FTHL6
应用：
WB,IHC,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P02794
规格：
目录价
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Description |
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FTH1 is the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. |
Specification |
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|---|---|
| Aliases | Ferritin heavy chain, Ferritin H subunit, Cell proliferation-inducing gene 15 protein, Ferritin heavy chain, N-terminally processed, FTH1, FTH, FTHL6 |
| Entrez GeneID | 2495 |
| Swissprot | P02794 |
| WB Predicted band size | 21.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This FTH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the C-terminal region of human FTH1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
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All lanes : Anti-FTH1 Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of FTH1 Antibody (C-term) (Cat. #P34205) in A2058 cell line lysates (35ug/lane). FTH1 (arrow) was detected using the purified Pab.
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Flow cytometric analysis of HepG2 cells using FTH1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma with FTH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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