Mouse Monoclonal Antibody to Myc Tag
货号：
P34299
别名：
Tag from c-Myc protein
应用：
WB
反应种属：
抗体类型：
Primary antibody
Swissprot：
规格：
目录价
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Description |
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Epitope tags consisting of short sequences recognized by well-characterizated monoclonal antibodies have been widely used in the study of protein expression in various systems. The HA tag (YPYDVPDYA) and Myc Tag (AEEQKLISEEDLLRKRREQLKHKLE), recognized by monoclonal antibody clones 12CA5 and 9E10, respectively, are illustrative examples. Abgent's anti-Myc monoclonal antibody (Clone 9E10) provides a simple solution to detect the expression of a Myc-tagged protein in cells. |
Specification |
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|---|---|
| Aliases | Tag from c-Myc protein |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Immunogen | KLH conjugated synthetic peptide encoding c-Myc tag (AEEQKLISEEDLLRKRREQLKHKLE) was used as antigen. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
Product Image
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Anti-Myc Tag Antibody at 1:2000 dilution + 12 tag recombinant protein lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45-50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of 12-tag protein lysate, using Myc Tag Antibody(Cat. #P34299). P34299 was diluted at 1:2000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 20ug.
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