Rabbit Polyclonal Antibody to IL12_2
货号：
P34310
别名：
Interleukin-12 receptor subunit beta-2, IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, IL-12RB2, IL12RB2
应用：
WB,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q99665
规格：
目录价
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Description |
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The protein encoded by this gene is a type I transmembrane protein identified as a subunit of the interleukin 12 receptor complex. The coexpression of this and IL12RB1 proteins was shown to lead to the formation of high-affinity IL12 binding sites and reconstitution of IL12 dependent signaling. The expression of this gene is up-regulated by interferon gamma in Th1 cells, and plays a role in Th1 cell differentiation. The up-regulation of this gene is found to be associated with a number of infectious diseases, such as Crohn's disease and leprosy, which is thought to contribute to the inflammatory response and host defense. |
Specification |
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| Aliases | Interleukin-12 receptor subunit beta-2, IL-12 receptor subunit beta-2, IL-12R subunit beta-2, IL-12R-beta-2, IL-12RB2, IL12RB2 |
| Entrez GeneID | 3595 |
| Swissprot | Q99665 |
| WB Predicted band size | 97.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This IL12_2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 756-783 amino acids from the C-terminal region of human IL12_2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000 |
| FCM | 1/10-1/50 |
Product Image
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All lanes : Anti-IL12RB2 Antibody (C-term) at 1:2000 dilution Lane 1: THP-1 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 97 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing A431 cells stained with P34310 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34310, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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