Mouse Monoclonal Antibody to SUFU
货号：
P34348
别名：
Suppressor of fused homolog, SUFUH, SUFU
应用：
WB
反应种属：
Human, African Green Mouseonkey, Mouse
抗体类型：
Primary antibody
Swissprot：
Q9UMX1
规格：
目录价
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Description |
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Negative regulator in the hedgehog signaling pathway. Down-regulates GLI1-mediated transactivation of target genes. Part of a corepressor complex that acts on DNA-bound GLI1. May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein. Negative regulator of beta-catenin signaling. Regulates the formation of either the repressor form (GLI3R) or the activator form (GLI3A) of the full length form of GLI3 (GLI3FL). GLI3FL is complexed with SUFU in the cytoplasm and is maintained in a neutral state. Without the Hh signal, the SUFU- GLI3 complex is recruited to cilia, leading to the efficient processing of GLI3FL into GLI3R. When Hh signaling is initiated, SUFU dissociates from GLI3FL and the latter translocates to the nucleus, where it is phosphorylated, destabilized, and converted to a transcriptional activator (GLI3A). Required for the proper formation of hair follicles and the control of epidermal differentiation (By similarity). |
Specification |
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|---|---|
| Aliases | Suppressor of fused homolog, SUFUH, SUFU |
| Entrez GeneID | 51684 |
| Swissprot | Q9UMX1 |
| WB Predicted band size | 53.9kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, African Green Mouseonkey, Mouse |
| Immunogen | This SUFU antibody is generated from a mouse immunized with a recombinant protein of human SUFU. |
Application |
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|---|---|
| WB | 1/2000 |
Product Image
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All lanes : Anti-SUFU Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: COS-7 whole cell lysate Lane 3: LNCap whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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P34348 staining SUFU in mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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