Rabbit Polyclonal Antibody to RPS2
货号：
P34368
别名：
40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3, RPS2, RPS4
应用：
WB,IF,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P15880
规格：
目录价
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Specification |
|
|---|---|
| Aliases | 40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3, RPS2, RPS4 |
| Entrez GeneID | 6187 |
| Swissprot | P15880 |
| WB Predicted band size | 31.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RPS2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 248-379 amino acids from human RPS2. |
Application |
|
|---|---|
| WB | 1/2000 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-RPS2 Antibody (C-Term) at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: A549 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling RPS2 with P34368 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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Overlay histogram showing Hela cells stained with P34368(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34368, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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