Mouse Monoclonal Antibody to GNMT
货号：
P34380
别名：
Glycine N-methyltransferase, 2.1.1.20, GNMT
应用：
WB,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q14749
规格：
目录价
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Description |
|---|
Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. |
Specification |
|
|---|---|
| Aliases | Glycine N-methyltransferase, 2.1.1.20, GNMT |
| Entrez GeneID | 27232 |
| Swissprot | Q14749 |
| WB Predicted band size | 32.7kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GNMT antibody is generated from a mouse immunized with a recombinant protein between 1-295 amino acids from the human GNMT. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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Anti-GNMT Antibody (C-term) at 1:4000 dilution + Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2 OS cells stained with P34380(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34380, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NH174309) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG2a (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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