Rabbit Polyclonal Antibody to OVGP1 (N-Term)
货号：
P34360
别名：
Oviduct-specific glycoprotein, Estrogen-dependent oviduct protein, Mucin-9, Oviductal glycoprotein, Oviductin, OVGP1, MUC9, OGP
应用：
WB,IHC,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q12889
规格：
目录价
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Description |
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Binds to oocyte zona pellucida in vivo. May play a role in the fertilization process and/or early embryonic development. |
Specification |
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|---|---|
| Aliases | Oviduct-specific glycoprotein, Estrogen-dependent oviduct protein, Mucin-9, Oviductal glycoprotein, Oviductin, OVGP1, MUC9, OGP |
| Entrez GeneID | 5016 |
| Swissprot | Q12889 |
| WB Predicted band size | 75.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This OVGP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 107-137 amino acids from human OVGP1. |
Application |
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|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-OVGP1 Antibody (N-Term) at 1:2000 dilution Lane 1: human placenta lysate Lane 2: human uterus lysate Lane 3: human ovary lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 75 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with P34360(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34360, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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P34360 staining OVGP1 in human fallopian tube tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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