Rabbit Polyclonal Antibody to DBH (N-term P42)
货号：
P34386
别名：
Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, Soluble dopamine beta-hydroxylase, DBH
应用：
WB,IF
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P09172
规格：
目录价
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Description |
|---|
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq]. |
Specification |
|
|---|---|
| Aliases | Dopamine beta-hydroxylase, Dopamine beta-monooxygenase, Soluble dopamine beta-hydroxylase, DBH |
| Entrez GeneID | 1621 |
| Swissprot | P09172 |
| WB Predicted band size | 69.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This DBH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 27-56 amino acids from the N-terminal region of human DBH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/2000 |
| IF | 1/25 |
Product Image
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All lanes : Anti-DBH Antibody (N-term P42) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: mouse adrenal gland lysate Lane 3: rat adrenal gland lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling DBH with P34386 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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