Rabbit Polyclonal Antibody to BIRC6
货号：
P34435
别名：
Baculoviral IAP repeat-containing protein 6, 6.3.2.-, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE, Ubiquitin-conjugating BIR domain enzyme apollon, APOLLON, BIRC6, KIAA1289
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q9NR09
规格：
目录价
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Description |
|---|
Anti-apoptotic protein which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase. Has an unusual ubiquitin conjugation system in that it could combine in a single polypeptide, ubiquitin conjugating (E2) with ubiquitin ligase (E3) activity, forming a chimeric E2/E3 ubiquitin ligase. Its tragets include CASP9 and DIABLO/SMAC. Acts as an inhibitor of CASP3, CASP7 and CASP9. Important regulator for the final stages of cytokinesis. Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification. |
Specification |
|
|---|---|
| Aliases | Baculoviral IAP repeat-containing protein 6, 6.3.2.-, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE, Ubiquitin-conjugating BIR domain enzyme apollon, APOLLON, BIRC6, KIAA1289 |
| Entrez GeneID | 57448 |
| Swissprot | Q9NR09 |
| WB Predicted band size | 530.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This BIRC6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4810-4844 amino acids from human BIRC6. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-BIRC6 Antibody (C-Term) at 1:1000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 530 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with P34435(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34435, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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