Rabbit Polyclonal Antibody to INTS3
货号：
P34431
别名：
Integrator complex subunit 3, Int3, SOSS complex subunit A, Sensor of single-strand DNA complex subunit A, SOSS-A, Sensor of ssDNA subunit A, INTS3, C1orf193, C1orf60
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q68E01
规格：
目录价
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Description |
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Component of the Integrator complex. The Integrator complex is involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. |
Specification |
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|---|---|
| Aliases | Integrator complex subunit 3, Int3, SOSS complex subunit A, Sensor of single-strand DNA complex subunit A, SOSS-A, Sensor of ssDNA subunit A, INTS3, C1orf193, C1orf60 |
| Entrez GeneID | 65123 |
| Swissprot | Q68E01 |
| WB Predicted band size | 118.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This INTS3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 518-551 amino acids from the Central region of human INTS3. |
Application |
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|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-INTS3 Antibody (Center) at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 118 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with P34431(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34431, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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