Rabbit Polyclonal Antibody to TOMM40 (N-Term)
货号：
P34437
别名：
Mitochondrial import receptor subunit TOM40 homolog, Protein Haymaker, Translocase of outer membrane 40 kDa subunit homolog, p38.5, TOMM40, C19orf1, PEREC1, TOM40
应用：
WB,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
O96008
规格：
目录价
在线咨询
Description |
|---|
Channel-forming protein essential for import of protein precursors into mitochondria. |
Specification |
|
|---|---|
| Aliases | Mitochondrial import receptor subunit TOM40 homolog, Protein Haymaker, Translocase of outer membrane 40 kDa subunit homolog, p38.5, TOMM40, C19orf1, PEREC1, TOM40 |
| Entrez GeneID | 10452 |
| Swissprot | O96008 |
| WB Predicted band size | 37.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This TOMM40 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 22-56 amino acids from human TOMM40. |
Application |
|
|---|---|
| WB | 1/2000 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
-
All lanes : Anti-TOMM40 Antibody (N-Term) at 1:2000 dilution Lane 1: Human brain lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: Hut78 whole cell lysate Lane 5: Hela whole cell lysate Lane 6: A431 whole cell lysate Lane 7: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
-
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling TOMM40 with P34437 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
-
Overlay histogram showing HeLa cells stained with P34437(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34437, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
鄂公网安备42018502007531号
