Rabbit Polyclonal Antibody to ST8SIA1 (N-Term)
货号：
P34442
别名：
Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, 2.4.99.8, Alpha-2,8-sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, Sialyltransferase 8A, SIAT8-A, Sialyltransferase St8Sia I, ST8SiaI, ST8SIA1, SIAT8, SIAT8A
应用：
WB,FCM
反应种属：
Human, Rat
抗体类型：
Primary antibody
Swissprot：
Q92185
规格：
目录价
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Description |
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Involved in the production of gangliosides GD3 and GT3 from GM3; gangliosides are a subfamily of complex glycosphinglolipds that contain one or more residues of sialic acid. |
Specification |
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| Aliases | Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, 2.4.99.8, Alpha-2,8-sialyltransferase 8A, Ganglioside GD3 synthase, Ganglioside GT3 synthase, Sialyltransferase 8A, SIAT8-A, Sialyltransferase St8Sia I, ST8SiaI, ST8SIA1, SIAT8, SIAT8A |
| Entrez GeneID | 6489 |
| Swissprot | Q92185 |
| WB Predicted band size | 40.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | This ST8SIA1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 53-84 amino acids from human ST8SIA1. |
Application |
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|---|---|
| WB | 1/1000-1/2000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-ST8SIA1 Antibody (N-Term) at 1:1000-1:2000 dilution Lane 1: A2058 whole cell lysate Lane 2: A375 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing A2058 cells stained with P34442(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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