Rabbit Polyclonal Antibody to CHRNE
货号：
P34478
别名：
Acetylcholine receptor subunit epsilon, CHRNE, ACHRE
应用：
WB,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q04844
规格：
目录价
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Description |
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After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. |
Specification |
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| Aliases | Acetylcholine receptor subunit epsilon, CHRNE, ACHRE |
| Entrez GeneID | 1145 |
| Swissprot | Q04844 |
| WB Predicted band size | 54.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This CHRNE antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 409-443 amino acids from the Central region of human CHRNE. |
Application |
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|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-CHRNE Antibody (Center) at 1:2000 dilution Lane 1: Human skeletal muslce lysate Lane 2: Mouse skeletal muscle lysate Lane 3: Rat skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing HepG2 cells stained with P34478(green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34478, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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