Rabbit Polyclonal Antibody to LZIC
货号：
P34461
别名：
Protein LZIC, Leucine zipper and CTNNBIP1 domain-containing protein, Leucine zipper and ICAT homologous domain-containing protein, LZIC
应用：
WB,IF,FCM
反应种属：
Human, Rat
抗体类型：
Primary antibody
Swissprot：
Q8WZA0
规格：
目录价
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Description |
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LZIC belongs to the CTNNBIP1 family. It is ubiquitously expressed with highest levels in kidney. LZIC is up-regulated in several cases of gastric cancers. |
Specification |
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|---|---|
| Aliases | Protein LZIC, Leucine zipper and CTNNBIP1 domain-containing protein, Leucine zipper and ICAT homologous domain-containing protein, LZIC |
| Entrez GeneID | 84328 |
| Swissprot | Q8WZA0 |
| WB Predicted band size | 21.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | This LZIC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-109 amino acids from the Central region of human LZIC. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/2000 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-LZIC Antibody (Center) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling LZIC with P34461 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus and Cytoplasm staining on Hela cell line. The nuclear counter stain is DAPI (blue).
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Overlay histogram showing Hela cells stained with P34461(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34461, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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