Rabbit Polyclonal Antibody to TCIRG1
货号：
P34502
别名：
V-type proton ATPase 116 kDa subunit a isoform 3, V-ATPase 116 kDa isoform a3, Osteoclastic proton pump 116 kDa subunit, OC-116 kDa, OC116, T-cell immune regulator 1, T-cell immune response cDNA7 protein, TIRC7, Vacuolar proton translocating ATPase 116 kD
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q13488
规格：
目录价
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Description |
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Part of the proton channel of V-ATPases (By similarity). Seems to be directly involved in T-cell activation. |
Specification |
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| Aliases | V-type proton ATPase 116 kDa subunit a isoform 3, V-ATPase 116 kDa isoform a3, Osteoclastic proton pump 116 kDa subunit, OC-116 kDa, OC116, T-cell immune regulator 1, T-cell immune response cDNA7 protein, TIRC7, Vacuolar proton translocating ATPase 116 kDa subunit a isoform 3, TCIRG1, ATP6N1C, ATP6V0A3 |
| Entrez GeneID | 10312 |
| Swissprot | Q13488 |
| WB Predicted band size | 93.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This TCIRG1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 668-702 amino acids from the human region of human TCIRG1. |
Application |
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| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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Anti-TCIRG1 Antibody (C-Term) at 1:2000 dilution + EL4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2 OS cells stained with P34502(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34502, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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