Rabbit Polyclonal Antibody to MYL2
货号：
P34486
别名：
Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, MLC-2, MLC-2v, MYL2
应用：
WB,IHC,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P10916
规格：
目录价
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Specification |
|
|---|---|
| Aliases | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform, MLC-2, MLC-2v, MYL2 |
| Entrez GeneID | 4633 |
| Swissprot | P10916 |
| WB Predicted band size | 18.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This MYL2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 42-75 amino acids from the Central region of human MYL2. |
Application |
|
|---|---|
| WB | 1/2000 |
| IHC | 1/250-1/1000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-MYL2 Antibody (Center) at 1:2000 dilution Lane 1: Human heart lysate Lane 2: Human skeletal muslce lysate Lane 3: Mouse heart lysate Lane 4: Rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2 OS cells stained with P34486(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34486, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using P34486 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded human heart tissue using P34486 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded Human Myocardium section using Pink1(Cat#P34486). P34486 was diluted at 1:250 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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