Rabbit Polyclonal Antibody to DUX4
货号：
P34495
别名：
Double homeobox protein 4 {ECO:0000312|HGNC:HGNC:50800}, Double homeobox protein 10 {ECO:0000312|EMBL:AAK915091}, DUX4 (<a href="http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=50800" target="_blank">HGNC:50800</a>), DUX10
应用：
WB,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q9UBX2
规格：
目录价
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Description |
|---|
DUX4 may be involved in transcriptional regulation. |
Specification |
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|---|---|
| Aliases | Double homeobox protein 4 {ECO:0000312|HGNC:HGNC:50800}, Double homeobox protein 10 {ECO:0000312|EMBL:AAK915091}, DUX4 (HGNC:50800), DUX10 |
| Entrez GeneID | 100288687 |
| Swissprot | Q9UBX2 |
| WB Predicted band size | 44.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DUX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 246-275 amino acids from the Central region of human DUX4. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/10-1/50 |
Product Image
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Anti-DUX4 Antibody (Center) at 1:2000 dilution + Human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing U-2 OS cells stained with P34495(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34495, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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