Rabbit Polyclonal Antibody to ATP1A4 (N-Term)
货号：
P34484
别名：
Sodium/potassium-transporting ATPase subunit alpha-4, Na(+)/K(+) ATPase alpha-4 subunit, 3.6.3.9, Sodium pump subunit alpha-4, ATP1A4, ATP1AL2
应用：
WB,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q13733
规格：
目录价
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Description |
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This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Plays a role in sperm motility. |
Specification |
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|---|---|
| Aliases | Sodium/potassium-transporting ATPase subunit alpha-4, Na(+)/K(+) ATPase alpha-4 subunit, 3.6.3.9, Sodium pump subunit alpha-4, ATP1A4, ATP1AL2 |
| Entrez GeneID | 480 |
| Swissprot | Q13733 |
| WB Predicted band size | 114.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ATP1A4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12-46 amino acids from the human region of human ATP1A4. |
Application |
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|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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Anti-ATP1A4 Antibody (N-Term) at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 114 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing HeLa cells stained with P34484(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34484, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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