Rabbit Polyclonal Antibody to CKS2 (N-Term)
货号：
P34507
别名：
Cyclin-dependent kinases regulatory subunit 2, CKS-2, CKS2
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
P33552
规格：
目录价
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Description |
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Binds to the catalytic subunit of the cyclin dependent kinases and is essential for their biological function. |
Specification |
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|---|---|
| Aliases | Cyclin-dependent kinases regulatory subunit 2, CKS-2, CKS2 |
| Entrez GeneID | 1164 |
| Swissprot | P33552 |
| WB Predicted band size | 9.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This CKS2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 6-40 amino acids from the human region of human CKS2. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-CKS2 Antibody (N-Term) at 1:2000 dilution Lane 1: Caco2 whole cell lysate Lane 2: RPMI 8226 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 10 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing HeLa cells stained with P34507(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34507, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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