Rabbit Polyclonal Antibody to COL14A1
货号：
P34514
别名：
Collagen alpha-1(XIV) chain, Undulin, COL14A1 (<a href="http://www.genenames.org/cgi-bin/gene_symbol_report?hgnc_id=2191" target="_blank">HGNC:2191</a>), UND
应用：
WB,IHC,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q05707
规格：
目录价
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Description |
|---|
Plays an adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors (By similarity). |
Specification |
|
|---|---|
| Aliases | Collagen alpha-1(XIV) chain, Undulin, COL14A1 (HGNC:2191), UND |
| Entrez GeneID | 7373 |
| Swissprot | Q05707 |
| WB Predicted band size | 193.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This COL14A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 643-677 amino acids from the Central region of human COL14A1. |
Application |
|
|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/25 |
Product Image
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Anti-COL14A1 Antibody (Center) at 1:2000 dilution + Human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 194 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with P34514(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34514, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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P34514 staining COL14A1 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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