Rabbit Polyclonal Antibody to gamma Tubulin
货号：
P34527
别名：
Tubulin gamma-2 chain, Gamma-2-tubulin, Tubg2
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q8VCK3
规格：
目录价
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Description |
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Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, centrosome duplication and spindle formation (By similarity). |
Specification |
|
|---|---|
| Aliases | Tubulin gamma-2 chain, Gamma-2-tubulin, Tubg2 |
| Entrez GeneID | 103768 |
| Swissprot | Q8VCK3 |
| WB Predicted band size | 51.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This gamma Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 400-434 amino acids from the mouse region of mouse gamma Tubulin. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-gamma Tubulin Antibody at 1:2000 dilution Lane 1: Mouse brain lysate Lane 2: Mouse liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing C2C12 cells stained with P34527(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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