Rabbit Polyclonal Antibody to beta II Tubulin
货号：
P34526
别名：
Tubulin beta-2A chain, Tubb2a, Tubb2
应用：
WB,IF,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q7TMM9
规格：
目录价
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Description |
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Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity). |
Specification |
|
|---|---|
| Aliases | Tubulin beta-2A chain, Tubb2a, Tubb2 |
| Entrez GeneID | 22151 |
| Swissprot | Q7TMM9 |
| WB Predicted band size | 49.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This beta II Tubulin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 194-225 amino acids from the mouse region of mouse beta II Tubulin. |
Application |
|
|---|---|
| WB | 1/2000 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-beta II Tubulin Antibody at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Mouse brain lysate Lane 4: Human brain lysate Lane 5: Hela whole cell lysate Lane 6: A431 whole cell lysate Lane 7: 293 whole cell lysate Lane 8: MCF-7 whole cell lysate Lane 9: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (mouse myoblast cell line) cells labeling beta II Tubulin with P34526 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoskeleton staining on C2C12 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
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Overlay histogram showing C2C12 cells stained with P34526(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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