Rabbit Polyclonal Antibody to IFITM5
货号：
P34544
别名：
Interferon-induced transmembrane protein 5, Bone-restricted interferon-induced transmembrane protein-like protein, BRIL, Dispanin subfamily A member 1, DSPA1, IFITM5
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
A6NNB3
规格：
目录价
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Description |
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Plays a role in bone mineralization (By similarity). |
Specification |
|
|---|---|
| Aliases | Interferon-induced transmembrane protein 5, Bone-restricted interferon-induced transmembrane protein-like protein, BRIL, Dispanin subfamily A member 1, DSPA1, IFITM5 |
| Entrez GeneID | 387733 |
| Swissprot | A6NNB3 |
| WB Predicted band size | 14.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This IFITM5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-83 amino acids of human IFITM5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-IFITM5 Antibody (Center) at 1:4000 dilution Lane 1: T47D whole cell lysate Lane 2: PC-3 whole cell lysate Lane 3: SW480 whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: PANC-1 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing A431 cells stained with P34544(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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