Rabbit Polyclonal Antibody to GPX7
货号：
P34538
别名：
Glutathione peroxidase 7, GPx-7, GSHPx-7, 1.11.1.9, CL683, GPX7, GPX6
应用：
WB,IHC,IF,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q96SL4
规格：
目录价
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Description |
|---|
It protects esophageal epithelia from hydrogen peroxide- induced oxidative stress. It suppresses acidic bile acid-induced reactive oxigen species (ROS) and protects against oxidative DNA damage and double-strand breaks. |
Specification |
|
|---|---|
| Aliases | Glutathione peroxidase 7, GPx-7, GSHPx-7, 1.11.1.9, CL683, GPX7, GPX6 |
| Entrez GeneID | 2882 |
| Swissprot | Q96SL4 |
| WB Predicted band size | 21.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This GPX7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 73-107 amino acids from the Central region of human GPX7. |
Application |
|
|---|---|
| WB | 1/2000 |
| IHC | 1/1000 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-GPX7 Antibody (Center) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: THP-1 whole cell lysate Lane 3: RPMI 8226 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2OS cells labeling GPX7 with P34538 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).
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Overlay histogram showing U-2 OS cells stained with P34538(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34538, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Immunohistochemical analysis of paraffin-embedded human liver tissue using P34538 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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