Rabbit Polyclonal Antibody to NTF3
货号：
P34574
别名：
Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor, NTF3
应用：
WB,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P20783
规格：
目录价
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Description |
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NTF3 is a member of the neurotrophin family that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. |
Specification |
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|---|---|
| Aliases | Neurotrophin-3, NT-3, HDNF, Nerve growth factor 2, NGF-2, Neurotrophic factor, NTF3 |
| Entrez GeneID | 4908 |
| Swissprot | P20783 |
| WB Predicted band size | 29.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This NTF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 152-184 amino acids from the C-terminal region of human NTF3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| FCM | 1/25 |
Product Image
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Anti-NTF3 Antibody (C-term) at 1:2000 dilution + NTF3 protein whole cell lysate Lysates/proteins at 20 ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 13. 5 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Anti-NTF3 Antibody (C-term) at 1:2000 dilution + Human ovary lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 29 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Hela cells stained with P34574(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34574, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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