Rabbit Polyclonal Antibody to ADA
货号：
P34588
别名：
Adenosine deaminase, Adenosine aminohydrolase, ADA, ADA1
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
P00813
规格：
目录价
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Description |
|---|
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. |
Specification |
|
|---|---|
| Aliases | Adenosine deaminase, Adenosine aminohydrolase, ADA, ADA1 |
| Entrez GeneID | 100 |
| Swissprot | P00813 |
| WB Predicted band size | 40.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ADA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human ADA. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/10-1/50 |
Product Image
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All lanes : Anti-ADA Antibody (C-term) at 1:1000 dilution Lane 1: MOLT-4 whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing Jurkat cells stained with P34588(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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