Rabbit Polyclonal Antibody to RS18
货号：
P34602
别名：
40S ribosomal protein S18, Ke-3, Ke3, RPS18, D6S218E
应用：
WB,IF,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P62269
规格：
目录价
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Description |
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Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. |
Specification |
|
|---|---|
| Aliases | 40S ribosomal protein S18, Ke-3, Ke3, RPS18, D6S218E |
| Entrez GeneID | 6222 |
| Swissprot | P62269 |
| WB Predicted band size | 17.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This RS18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of human RS18. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-RS18 Antibody (Center) at 1:1000 dilution Lane 1: U-251 MG whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: THP-1 whole cell lysate Lane 4: Daudi whole cell lysate Lane 5: Mouse spleen lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 18 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2OS cells labeling RPS18 with P34602 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on U-2OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/500 dilution (red). The nuclear counter stain is DAPI (blue).
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Overlay histogram showing U-2 OS cells stained with P34602(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34602, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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