Rabbit Polyclonal Antibody to CDC25A (S124)
货号:
P34629
别名:
M-phase inducer phosphatase 1, 3.1.3.48, Dual specificity phosphatase Cdc25A, CDC25A
应用:
WB,IHC
反应种属:
Human, Mouse, Rat
抗体类型:
Primary antibody
Swissprot:
P30304
规格:
目录价
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Description |
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Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro. |
Specification |
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Aliases | M-phase inducer phosphatase 1, 3.1.3.48, Dual specificity phosphatase Cdc25A, CDC25A |
Entrez GeneID | 993 |
Swissprot | P30304 |
WB Predicted band size | 59.1kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human. |
Application |
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WB | 1/1000 |
IHC | 1/100 |
Product Image
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All lanes : Anti-CDC25A(S124) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: A431 whole cell lysate Lane 4: NIH/3T3 whole cell lysate Lane 5: Rat testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of P34629 on paraffin-embedded Human breast carcinoma tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of P34629 on paraffin-embedded Human tonsil tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.