Rabbit Polyclonal Antibody to SPNS2 (N-term)
货号：
P34593
别名：
Protein spinster homolog 2, SPNS2
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q8IVW8
规格：
目录价
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Description |
|---|
Sphingolipid transporter required for migration of myocardial precursors. Transports sphingosine 1-phosphate (S1P), a secreted lipid mediator that plays critical roles in cardiovascular, immunological, and neural development and function. Mediates the export of S1P from cells in the extraembryonic yolk syncytial layer (YSL), thereby regulating myocardial precursor migration. |
Specification |
|
|---|---|
| Aliases | Protein spinster homolog 2, SPNS2 |
| Entrez GeneID | 124976 |
| Swissprot | Q8IVW8 |
| WB Predicted band size | 58.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This SPNS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 68-94 amino acids from the N-terminal region of human SPNS2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
Product Image
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Anti-SPNS2 Antibody (N-term) at 1:1000 dilution + SK-OV-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SPNS2 Antibody (N-term) at 1:1000 dilution Lane 1: Human heart lysate Lane 2: Human kidney lysate Lane 3: Human skeletal muscle lysate Lane 4: SK-BR-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing SK-OV-3 cells stained with P34593(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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