Rabbit Polyclonal Antibody to ENASE
货号：
P34659
别名：
Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE
应用：
WB,IHC,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q8NFI3
规格：
目录价
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Description |
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Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) is involved in the processing of free oligosaccharides in the cytosol. |
Specification |
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|---|---|
| Aliases | Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE |
| Entrez GeneID | 64772 |
| Swissprot | Q8NFI3 |
| WB Predicted band size | 84.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
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All lanes : Anti-ENASE Antibody (Center) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: human brain tissue lysate Lane 3: mouse thymus tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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ENASE Antibody (Center) (Cat. #P34659) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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ENASE Antibody (Center) (Cat. #P34659) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ENASE Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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