Rabbit Polyclonal Antibody to ALOXE3
货号：
P34705
别名：
Hydroperoxide isomerase ALOXE3, Epidermis-type lipoxygenase 3, Epidermal LOX-3, e-LOX-3, eLOX-3, Hydroperoxy icosatetraenoate dehydratase, ALOXE3
应用：
WB,IHC
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q9BYJ1
规格：
目录价
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Description |
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ALOXE3 is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. |
Specification |
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| Aliases | Hydroperoxide isomerase ALOXE3, Epidermis-type lipoxygenase 3, Epidermal LOX-3, e-LOX-3, eLOX-3, Hydroperoxy icosatetraenoate dehydratase, ALOXE3 |
| Entrez GeneID | 59344 |
| Swissprot | Q9BYJ1 |
| WB Predicted band size | 80.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ALOXE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-252 amino acids from the Central region of human ALOXE3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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| WB | 1/1000 |
| IHC | 1/500 |
Product Image
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All lanes : Anti-ALOXE3 Antibody (Center) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: HACAT whole cell lysate Lane 3: A2058 whole cell lysate Lane 4: T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 81 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human breast tissue using P34705 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded human skin tissue using P34705 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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