Rabbit Polyclonal Antibody to SIM1 (N-term)
货号：
P34722
别名：
Single-minded homolog 1, Class E basic helix-loop-helix protein 14, bHLHe14, SIM1, BHLHE14
应用：
WB,IHC
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P81133
规格：
目录价
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Description |
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SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome. |
Specification |
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|---|---|
| Aliases | Single-minded homolog 1, Class E basic helix-loop-helix protein 14, bHLHe14, SIM1, BHLHE14 |
| Entrez GeneID | 6492 |
| Swissprot | P81133 |
| WB Predicted band size | 85.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SIM1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/2000 |
| IHC | 1/250 |
Product Image
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Anti-SIM1 Antibody (N-term) at 1:2000 dilution + A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of P34722 on paraffin-embedded Human kidney tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
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Immunohistochemical analysis of P34722 on paraffin-embedded Human brain tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.
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