Rabbit Polyclonal Antibody to COL8A2
货号：
P34720
别名：
Collagen alpha-2(VIII) chain, Endothelial collagen, COL8A2
应用：
WB,IHC
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P25067
规格：
目录价
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Description |
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This gene encodes the alpha 2 chain of type VIII collagen. The protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. |
Specification |
|
|---|---|
| Aliases | Collagen alpha-2(VIII) chain, Endothelial collagen, COL8A2 |
| Entrez GeneID | 1296 |
| Swissprot | P25067 |
| WB Predicted band size | 67.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This COL8A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 571-599 amino acids from the C-terminal region of human COL8A2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/2000 |
| IHC | 1/500 |
Product Image
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All lanes : Anti-COL8A2 Antibody (C-term) at 1:2000 dilution Lane 1: SH-SY5Y whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: Neuro-2a whole cell lysate Lane 4: C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue using AP11859B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded Human testis tissue using AP11859B performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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