Rabbit Polyclonal Antibody to PRK2
货号:
P34956
别名:
Serine/threonine-protein kinase N2, PKN gamma, Protein kinase C-like 2, Protein-kinase C-related kinase 2, PKN2, PRK2, PRKCL2
应用:
WB,IHC
反应种属:
Human, Mouse, Rat
抗体类型:
Primary antibody
Swissprot:
Q16513
规格:
目录价
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Description |
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Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK). |
Specification |
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Aliases | Serine/threonine-protein kinase N2, PKN gamma, Protein kinase C-like 2, Protein-kinase C-related kinase 2, PKN2, PRK2, PRKCL2 |
Entrez GeneID | 5586 |
Swissprot | Q16513 |
WB Predicted band size | 112.0kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This PRK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627-658 amino acids from the C-terminal region of human PRK2. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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WB | 1/1000-1/2000 |
IHC | 1/100-1/500 |
Product Image
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All lanes : Anti-hPRK2-E640 at 1:2000 dilution Lane 1: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M(H/L), Peroxidase conjugated at 1/2000 dilution. Observed band size : 130kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-PRK2 Pab (Cat. #P34956) in placenta lysate. PRK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.