Rabbit Polyclonal Antibody to LGR5 (N-term)
货号：
P35034
别名：
Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67
应用：
WB,IHC
反应种属：
Human, Mouse, Rat, Hamster
抗体类型：
Primary antibody
Swissprot：
O75473
规格：
目录价
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Description |
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Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. |
Specification |
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|---|---|
| Aliases | Leucine-rich repeat-containing G-protein coupled receptor 5, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, LGR5, GPR49, GPR67 |
| Entrez GeneID | 8549 |
| Swissprot | O75473 |
| WB Predicted band size | 100.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat, Hamster |
| Immunogen | This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human. |
Application |
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|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/500-1/1000 |
Product Image
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All lanes : Anti-LGR5 antibody (N-term) at 1:1000 dilution+ 3T3-L1 cell lysate Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 100kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of LGR5 Rabbit Brain tissue using P35034 performed on the Abcarta FAIP-30 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(Ready-to-use) for 15 min at room temperature. AmpSeeTM Detection Systems was used as the secondary antibody.
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Immunohistochemical analysis of LGR5 Rabbit Small intestine tissue using P35034 performed on the Abcarta FAIP-30 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(Ready-to-use) for 15 min at room temperature. AmpSeeTM Detection Systems was used as the secondary antibody.
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