Rabbit Polyclonal Antibody to MKK3(Phospho-Ser189)
货号：
P40140
别名：
MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3
应用：
WB,IHC,IF
反应种属：
Human,Mouse,Rat
抗体类型：
Primary antibody
Swissprot：
P46734
规格：
目录价
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Description |
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Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. |
References |
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| Wang W, et al. (2002) Mol Cell Biol ; 22(10): 3389-403. Raingeaud J, et al. (1996) Mol Cell Biol; 16(3): 1247-55. |
Specification |
|
|---|---|
| Aliases | MAP kinase kinase 3; MAP2K3; MAPK/ERK kinase 3; MAPKK 3; MEK3 |
| Entrez GeneID | 5606; |
| Swissprot | P46734 |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 189 (V-D-S(p)-V-A) derived from Human MKK3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IHC | 1/50-1/100 |
| IF | 1/100-1/200 |
Product Image
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Western blot analysis of extracts from Hela cells untreated or treated with PMA using MKK3(Phospho-Ser189) Antibody P40140.
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Western blot analysis of extracts from 3T3 cells, treated with Anisomycin or calf intestinal phosphatase (CIP), using MKK3 (Phospho-Ser189) Antibody P40140.
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Immunofluorescence staining of methanol-fixed Hela cells using MKK3(Phospho-Ser189) Antibody P40140.
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK3(Phospho-Ser189) Antibody P40140(left) or the same antibody preincubated with blocking peptide(right).
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